Venipuncture Steps (2022): Correct Order, Tips, Procedure with Picture | Virohan

9 Most Important Steps For Venipuncture

By Arushi Trivedi on 24 Aug 2022
2 mins read
Table Of Content

Venipuncture Steps

1. Test Report Form

  • A properly filled test requisition form is the first important step of blood sample analysis. 
  • It contains the patient identification and test detail information.  
  • It acts as a link between the physician and the laboratory. 
  • It significantly affects laboratory outcomes and ultimately patient safety. 
  • Collecting a sample from an incorrectly identified patient can lead to serious consequences such as misdiagnosis.

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2. Patient Preparation

  • The next important step is preparing the patient.  
  • It also includes taking the patient’s consent before starting the venipuncture
  • Putting the patient at ease helps in successful sample collection.

3. Patient Safety

  • Make sure you are aware of the health status/condition of the patient.  
  • It is also important for you to be aware of any known allergies or phobias of the patient.

4. Sanitation and Sterilization

  • The next point of prime importance is to not let any infection spread during venipuncture.  
  • All safety and sterilization protocols should be followed strictly.  
  • Hand sanitization should be performed in view of the patient. 
  • It is an important step in the venipuncture procedure.  
  • The site of venipuncture once cleaned with antiseptic should not be touched by bare hands thereafter. 
  • All equipment like collection tubes and needles should be checked before us

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5. Patient Positioning and Tourniquet Application

  • Positioning the patient correctly and applying the tourniquet are the next important steps to be followed in venipuncture.  
  • Most samples are drawn with the patient sitting up, while many patients prefer reclining chairs.  
  • If a patient is known to have vasovagal (when a patient faints in a stressful situation), then the lying down position should be preferred for sample collection.  
  • It is important to check that the hand is at a lower level than the arm during venipuncture.

6. Performing Venipuncture

  • A flash of blood in the hub of the syringe indicates successful entry into the vein. 
  • Blood is collected by slowly pulling the plunger.  
  • At this point, the tied tourniquet should be released.

7. Hematoma Formation Prevention

  • The needle should be retrieved smoothly and pressure should be applied at the site of collection only after the needle has been withdrawn.  
  • This helps prevent hematoma formation.

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8. Careful Waste Disposal

  • It is very important to ensure that all the sharps and other contaminated materials are properly discarded in specified containers or biohazard containers.  
  • Hands and the procedure area should be sanitized after waste disposal.

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9. Care While Transportation

  • Transport of specimens, if required, should be done timely and properly. 
  • Samples should be transported in sealed leak-proof bags/containers.

All these kept in mind lay the foundation of an effective venipuncture procedure.